Enhanced expression of trim14 gene suppressed Sindbis virus reproduction and modulated the transcription of a large number of genes of innate immunity

Immunol Res. 2015 Jul;62(3):255-62. doi: 10.1007/s12026-015-8653-1.


In the present research, we have studied an influence of enhanced expression TRIM14 on alphavirus Sindbis (SINV, Togaviridae family) infection. In the HEK293 cells transfected with human trim14 gene (HEK-trim14), SINV yield after infection was decreased 1000-10,000 times (3-4 lg of TCD50/ml) at 24 h p.i. and considerably less (1-2 lg of TCD50/ml) at 48 h p.i. Analysis of the expression of 43 genes directly or indirectly involved in innate immune machine in HEK-trim14 non-infected cells comparing with the control (non-transfected) HEK293 cells revealed that stable trim14 transfection in HEK293 cells caused increased transcription of 18 genes (ifna, il6 (ifnβ2), isg15, raf-1, NF-kB (nf-kb1, rela, nf-kb2, relb), grb2, grb3-3, traf3ip2, junB, c-myb, pu.1, akt1, tyk2, erk2, mek2) and lowered transcription of 3 genes (ifnγ, gata1, il-17a). The similar patterns of genes expression observe in SINV-infected non-transfected HEK293 cells. However, SINV infection of HEK-trim14 cells caused inhibition of the most interferon cascade genes as well as subunits of transcription factor NF-κB. Thus, stable enhanced expression of trim14 gene in cells activates the transcription of many immunity genes and suppresses the SINV reproduction, but SINV infection of HEK-trim14 cells promotes inhibition of some genes involved in innate immune system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alphavirus Infections / immunology*
  • Alphavirus Infections / virology
  • Carrier Proteins / biosynthesis
  • Carrier Proteins / genetics*
  • Cell Line
  • HEK293 Cells
  • Host-Pathogen Interactions / immunology
  • Humans
  • Immunity, Innate / genetics*
  • Intracellular Signaling Peptides and Proteins
  • Sindbis Virus / growth & development*
  • Sindbis Virus / immunology
  • Transfection
  • Tripartite Motif Proteins
  • Virus Replication / genetics*


  • Carrier Proteins
  • Intracellular Signaling Peptides and Proteins
  • TRIM14 protein, human
  • Tripartite Motif Proteins