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, 17 (3), 199-205

Genetic Differences of Three Pollicipes Mitella Populations Identified by PCR Analysis

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Genetic Differences of Three Pollicipes Mitella Populations Identified by PCR Analysis

Young-Jae Song et al. Dev Reprod.

Abstract

Genomic DNAs were extracted from the turtle leg (Pollicipes mitella, 1798) population of Tongyeong, Yeosu and Manjaedo located in the southern sea of Korea. The turtle leg population from Tongyeong (0.929) exhibited higher bandsharing values than did turtle leg from Manjaedo (0.852). The higher fragment sizes (>1,200 bp) are much more observed in the Yeosu population. The number of unique loci to each population and number of shared loci by the three populations, generated by PCR using 7 primers in the turtle leg (P. mitella) population of Tongyeong, Yeosu and Manjaedo. Genetic distances among different individuals of the Tongyeong population of the turtle leg (lane 1-07), Yeosu population of the turtle leg (lane 08-14) and Manjaedo population of the turtle leg (lane 15-21), respectively, were generated using the CLASSIFICATION option in Systat version 10 according to the bandsharing values and similarity matrix. The dendrogram, obtained by the seven decamer primers, indicated three genetic clusters: cluster 1 (TONGYEONG 01-TONGYEONG 07), cluster 2 (YEOSU 08-YEOSU 14), and cluster 3 (MANJEDO 15-MANJEDO 21). Tongyeong population could be evidently discriminated with the other two Yeosu and Manjaedo populations among three populations. The longest genetic distance (0.305) was found to exist between individuals' no. 02 of the Tongyeong population and no. 13 of the Yeosu population. It seems to the authors that this is a result of a high degree of inbreeding in narrow region for a long while.

Keywords: Genetic clusters; Genetic distance; Inbreeding; Manjaedo; Pollicipes mitella; Tongyeong; Turtle leg; Yeosu..

Figures

Fig. 1
Fig. 1
Genomic DNA samples isolated from three geographical Pollicipes mitella populations in Tongyeong, Yeosu and Manjaedo located in the southern sea of the Korean Peninsula.
Fig. 2
Fig. 2
Distribution of fragment sizes of Tongyeong, Yeosu and Manjaedo population of Pollicipes mitella. Solid lines: Tongyeong population. Dotted lines: Yeosu population. Thick dotted lines: Manjaedo population. The fragment numbers in each size interval have been computed from the pooled fragments obtained with all the primers. The higher fragment sizes (>1,200 bp) are much more observed in the Yeosu population.
Fig. 3
Fig. 3
PCR-based electrophoretic profiles of individual Pollicipes mitella. Each lane shows DNA samples extracted from 21 individuals. DNA isolated from Tongyeong population (lane 1–7), Yeosu population (lane 8–14) and Manjaedo population of P. mitella (lane 15–21) were amplified by decamer primers BION-45 (A), BION-52 (B), BION-55 (C), BION-58 (D), BION-67 (E), BION-76 (F) and BION-78 (G). The PCR products were separated by 1.4% agarose gel electrophoresis and detected by ethidium bromide staining. 100 bp ladder marker was utilized as a DNA molecular size marker.
Fig. 4
Fig. 4
Hierarchical dendrogram of genetic distances, obtained from three populations of Pollicipes mitella. The relatedness among different individuals in the P. mitella populations from Tongyeong, Yeosu and Manjaedo of the Korean peninsula were generated according to the bandsharing values and similarity matrix.

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