Isolation of Lactic Acid Bacteria Showing Antioxidative and Probiotic Activities from Kimchi and Infant Feces

J Microbiol Biotechnol. 2015 Sep;25(9):1568-77. doi: 10.4014/jmb.1501.01077.


The purpose of this study was to investigate lactic acid bacteria with antioxidative and probiotic activities isolated from Korean healthy infant feces and kimchi. Isolates A1, A2, S1, S2, and S3 were assigned to Lactobacillus sp. and isolates A3, A4, E1, E2, E3, and E4 were assigned to Leuconostoc sp. on the basis of their physiological properties and 16S ribosomal DNA sequence analysis. Most strains were confirmed as safe bioresources through nonhemolytic activities and non-production of harmful enzymes such as β-glucosidase, β- glucuronidase and tryptophanase. The 11 isolates showed different resistance to acid and bile acids. In addition, they exhibited antibacterial activity against foodborne bacteria, especially Bacillus cereus, Listeria monocytogenes, and Escherichia coli. Furthermore, all strains showed significantly high levels of hydrophobicity. The antioxidant effects of culture filtrates of the 11 strains included 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging capacity, 2.2'- azino-bis (2-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical cation scavenging activity, and superoxide dismutase activity. The results revealed that most of the culture filtrates have effective scavenging activity for DPPH and ABTS radicals. All strains appeared to have effective superoxide dismutase activity. In conclusion, the isolated strains A1, A3, S1, and S3 have significant probiotic activities applicable to the development of functional foods and health-related products. These strains might also contribute to preventing and controlling several diseases associated with oxidative stress, when used as probiotics.

Keywords: Antioxidant; Kimchi; Lactic acid bacteria; Probiotics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acids / toxicity
  • Antibiosis
  • Antioxidants / pharmacology*
  • Cluster Analysis
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Enzymes / analysis
  • Feces / microbiology*
  • Food Microbiology*
  • Hemolysin Proteins / analysis
  • Humans
  • Infant
  • Lactobacillus / classification
  • Lactobacillus / genetics
  • Lactobacillus / isolation & purification*
  • Lactobacillus / physiology
  • Leuconostoc / classification
  • Leuconostoc / genetics
  • Leuconostoc / isolation & purification*
  • Leuconostoc / physiology
  • Microbial Viability / drug effects
  • Phylogeny
  • Probiotics / pharmacology*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA


  • Acids
  • Antioxidants
  • DNA, Bacterial
  • DNA, Ribosomal
  • Enzymes
  • Hemolysin Proteins
  • RNA, Ribosomal, 16S