Leuconostoc rapi sp. nov., isolated from sous-vide-cooked rutabaga

Int J Syst Evol Microbiol. 2015 Aug;65(8):2586-2590. doi: 10.1099/ijs.0.000305. Epub 2015 May 7.


A Gram-stain-positive, ovoid, lactic acid bacterium, strain LMG 27676T, was isolated from a spoiled sous-vide-cooked rutabaga. 16S rRNA gene sequence analysis indicated that the novel strain belongs to the genus Leuconostoc, with Leuconostoc kimchii and Leuconostoc miyukkimchii as the nearest neighbours (99.1 and 98.8% 16S rRNA gene sequence similarity towards the type strain, respectively). Phylogenetic analysis of the 16S rRNA gene, multilocus sequence analysis of the pheS, rpoA and atpA genes, and biochemical and genotypic characteristics allowed differentiation of strain LMG 27676T from all established species of the genus Leuconostoc. Strain LMG 27676T ( = R-50029T = MHB 277T = DSM 27776T) therefore represents the type strain of a novel species, for which the name Leuconostoc rapi sp. nov. is proposed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • Brassica napus / microbiology*
  • Cooking
  • DNA, Bacterial / genetics
  • Finland
  • Food Microbiology*
  • Genes, Bacterial
  • Leuconostoc / classification*
  • Leuconostoc / genetics
  • Leuconostoc / isolation & purification
  • Molecular Sequence Data
  • Multilocus Sequence Typing
  • Nucleic Acid Hybridization
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Vegetables / microbiology


  • DNA, Bacterial
  • RNA, Ribosomal, 16S