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. 2015 Aug;82(4):289-91.
doi: 10.1016/j.diagmicrobio.2015.04.004. Epub 2015 Apr 24.

Molecular Diagnosis of Toxoplasmosis: Value of the Buffy Coat for the Detection of Circulating Toxoplasma Gondii

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Molecular Diagnosis of Toxoplasmosis: Value of the Buffy Coat for the Detection of Circulating Toxoplasma Gondii

Marie-Pierre Brenier-Pinchart et al. Diagn Microbiol Infect Dis. .

Abstract

Early detection of Toxoplasma tachyzoites circulating in blood using PCR is recommended for immunosuppressed patients at high risk for disseminated toxoplasmosis. Using a toxoplasmosis mouse model, we show that the sensitivity of detection is higher using buffy coat isolated from a large blood volume than using whole blood for this molecular monitoring.

Keywords: Buffy coat; Diagnosis; PCR; Toxoplasma gondii; Toxoplasmosis; Whole blood.

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