Protective effect of theophylline on renal functions in experimental pneumoperitoneum model

Ren Fail. 2015 Jul;37(6):1044-9. doi: 10.3109/0886022X.2015.1040706. Epub 2015 May 11.


Our objective in this experimental study is to research the effect of the intra-abdominal pressure which rises following pneumoperitoneum and whether Theophylline has a possible protective activity on this situation. In our study, 24 Wistar Albino rats were used. Rats were divided into two groups. The first group was set for only pneumoperitoneum model. The second group was given 15 mg/kg of Theophylline intraperitoneally before setting pneumoperitoneum model. Then urea, creatinine, cystatin-C, tissue and serum total antioxidant capacity, total oxidant capacity and oxidative stress index in two groups were measured and compared with each other. Apoptosis and histopathological conditions in the renal tissues were examined. The differences between the groups were analyzed with the Mann-Whitney U test. Results were considered significant at p < 0.05. No statistically significant difference was determined between tissue and serum averages in two groups in terms of TAS, TOS and OSI values (p > 0.05). The mean value of urea were similar in pneumoperitoneum and pneumoperitoneum + theophylline groups (p = 0.12). The mean cystatin-C value was 2.2 ± 0.3 µg/mL in pneumoperitoneum, 1.74 ± 0.33 µg/mL in pneumoperitoneum + theophylline (p = 0.002). According to our study, lower cystatin-C levels in the group, where Theophylline was given, are suggestive of lower renal injury in this group. However, this opinion is interrogated as there is no difference in terms of tissue and serum TAS, TOS, OSI and urea values between the groups.

Keywords: Laparoscopy; pneumoperitoneum; rat; renal function; theophylline.

Publication types

  • Comparative Study

MeSH terms

  • Acute Kidney Injury / prevention & control*
  • Animals
  • Biomarkers / blood
  • Biopsy, Needle
  • Creatine / blood
  • Cystatin C / blood
  • Disease Models, Animal
  • Immunohistochemistry
  • Injections, Intraperitoneal
  • Kidney Function Tests
  • Laparotomy
  • Male
  • Oxidative Stress / drug effects*
  • Pneumoperitoneum, Artificial / methods*
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Reperfusion Injury / prevention & control*
  • Sensitivity and Specificity
  • Theophylline / pharmacology*
  • Urea / blood


  • Biomarkers
  • Cystatin C
  • Urea
  • Theophylline
  • Creatine