Phytoactinopolyspora endophytica gen. nov., sp. nov., a halotolerant filamentous actinomycete isolated from the roots of Glycyrrhiza uralensis F

Int J Syst Evol Microbiol. 2015 Aug;65(8):2671-2677. doi: 10.1099/ijs.0.000322. Epub 2015 May 11.


A novel endophytic actinomycete, designated strain EGI 60009T, was isolated from the roots of Glycyrrhiza uralensis F. collected from Xinjiang Province, north-west China. The isolate was able to grow in the presence of 0-9% (w/v) NaCl. Strain EGI 60009T had particular morphological properties: the substrate mycelia fragmented into rod-like elements and aerial mycelia differentiated into short spore chains. ll-2, 6-Diaminopimelic acid was the cell-wall diamino acid and rhamnose, galactose and glucose were the cell-wall sugars. MK-9(H4) was the predominant menaquinone. The major fatty acids of strain EGI 60009T were iso-C15 : 0, anteiso-C15 : 0, anteiso-C17 : 0, iso-C17 : 0, iso-C17 : 1 and I/anteiso-C17 : 0 B. Mycolic acids were absent. The DNA G+C content of strain EGI 60009T was 70.4 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain EGI 60009T belongs to the family Jiangellaceae and formed a distinct clade in the phylogenetic tree. 16S rRNA gene sequence similarities between strain EGI 60009T and other members of the genera Jiangella and Haloactinopolyspora were 96.1-96.4 and 95.7-96.0%, respectively. Based on these results and supported by morphological, physiological and chemotaxonomic data and numerous phenotypic differences, a novel species of a new genus, Phytoactinopolyspora endophytica gen. nov., sp. nov., is proposed. The type strain of Phytoactinopolyspora endophytica is EGI 60009T ( = KCTC 29657T = CPCC204078T).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinomycetales / classification*
  • Actinomycetales / genetics
  • Actinomycetales / isolation & purification
  • Bacterial Typing Techniques
  • Base Composition
  • China
  • DNA, Bacterial / genetics
  • Diaminopimelic Acid / chemistry
  • Fatty Acids / chemistry
  • Glycyrrhiza uralensis / microbiology*
  • Molecular Sequence Data
  • Phylogeny*
  • Plant Roots / microbiology*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Vitamin K 2 / analogs & derivatives
  • Vitamin K 2 / chemistry


  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • menaquinone 9
  • Diaminopimelic Acid