Prevalence of Pf1-like (pro)phage genetic elements among Pseudomonas aeruginosa isolates

Virology. 2015 Sep;483:64-71. doi: 10.1016/j.virol.2015.04.008. Epub 2015 May 15.

Abstract

Pf1-like bacteriophages (family Inoviridae) of Pseudomonas aeruginosa can contribute to bacterial short term evolution and virulence. Here we examine Pf1-like (pro)phage diversity and prevalence among different P. aeruginosa isolates. Pf1-like prophages in sequenced genomes of P. aeruginosa were analyzed and grouped into four clades: Pf4, Pf5, Pf7 and Pf-LES. P. aeruginosa strains (n=241) were screened for the presence of universal (primers PfUa and PfUb) and specific Pf1-like genetic elements (Pf1, Pf4 and Pf5). More than half of the strains contained at least one Pf1-like genetic element (60%); universal elements were detected in 56% of the strains, Pf4 in 22%, Pf1 in 18% and Pf5 in 7%. Infectivity experiments confirmed that strains yielding PCR products with either universal or Pf4 specific primers can release infective virions. Based on the high prevalence of Pf1-like (pro)phages, it is necessary to further examine their involvement in P. aeruginosa virulence.

Keywords: Pf1-like phage; Prevalence; Pseudomonas aeruginosa prophage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cluster Analysis
  • DNA Primers / genetics
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Genetic Variation
  • Molecular Sequence Data
  • Phylogeny
  • Polymerase Chain Reaction
  • Prevalence
  • Prophages / genetics*
  • Pseudomonas Phages / genetics*
  • Pseudomonas aeruginosa / genetics*
  • Pseudomonas aeruginosa / virology*
  • Sequence Analysis, DNA
  • Sequence Homology

Substances

  • DNA Primers
  • DNA, Bacterial

Associated data

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