Demonstration of a new antinuclear antibody (anti-RA33) that is highly specific for rheumatoid arthritis

Arthritis Rheum. 1989 Dec;32(12):1515-20. doi: 10.1002/anr.1780321204.


Using immunoblot analysis with soluble nuclear extracts from HeLa cells, we identified autoantibodies to an antigen with a molecular weight of approximately 33,000 in 36% of 95 sera from rheumatoid arthritis patients, but in only 1 of 170 controls. The antigen, termed RA33, was resistant to DNase and RNase digestion but sensitive to proteinase K treatment. There was no discernible relation to other autoantibodies. Thus, this newly described autoantibody appears to be highly specific for rheumatoid arthritis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Antibodies, Antinuclear / analysis
  • Antibodies, Antinuclear / immunology*
  • Antibody Specificity
  • Arthritis, Rheumatoid / blood
  • Arthritis, Rheumatoid / immunology*
  • Autoantibodies / analysis
  • Female
  • Humans
  • Male
  • Middle Aged
  • Molecular Weight
  • Synovial Fluid / immunology


  • Antibodies, Antinuclear
  • Autoantibodies