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. 2015 May;22(3):340-50.
doi: 10.1016/j.sjbs.2015.02.005. Epub 2015 Feb 16.

Assessment of Genetic Diversity Among Faba Bean Genotypes Using Agro-Morphological and Molecular Markers

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Free PMC article

Assessment of Genetic Diversity Among Faba Bean Genotypes Using Agro-Morphological and Molecular Markers

Megahed H Ammar et al. Saudi J Biol Sci. .
Free PMC article

Abstract

Forty faba bean (Vicia faba L.) genotypes were evaluated for their agro-morphological performance and molecular diversity under Central Region of Saudi Arabia conditions during 2010-11 and 2011-12 seasons. Field performance results showed that faba genotypes exhibited a significant amount of variation for their agro-morphological studied parameters. Giza40 recorded the tallest genotype (139.5 cm), highest number of seeds per plants (100.8), and the highest seed yield per plant (70.8 g). The best performing genotypes were Giza40, FLIP03-014FB, Gazira1 and Goff1. Genetic variability among genotypes was determined using Sequence Related Amplified Polymorphism (SRAP) and Amplified Fragment Length Polymorphism (AFLP) markers. A total of 183 amplified fragments (alleles) and 1758 polymorphic fragments (bands) in SRAP and 202 alleles and 716 bands in AFLP were obtained using six SRAP and four AFLP primer combinations respectively. Polymorphism information content (PIC) values for AFLP and SRAP markers were higher than 0.8, indicating the existence of a considerable amount of genetic diversity among faba tested genotypes. The UPGMA based clustering of faba genotypes was largely based on origin and/or genetic background. Result of cluster analysis based on SRAP showed weak and not significant correlation while, it was highly significant based on AFLP analysis with agro-morphological characters (r = 0.01, p > 0.54 and r = 0.26, p < 0.004 respectively). Combined SRAP and AFLP markers proved to be significantly useful for genetic diversity assessment at molecular level. They exhibited high discrimination power, and were able to distinguish the faba bean genotypes with high efficiency and accuracy levels.

Keywords: AFLP; Faba bean; Molecular diversity; SRAP.

Figures

Fig. 1
Fig. 1
Two-dimensional ordination of 7 agro-morphological traits in faba bean genotypes on principal component axes. NP/P = number of pods/plant, NS/P = number of seeds/plant, SY/P = number of seeds/plant, PH = plant height, NB/P = number of branches/plant, ND-50 = number of days to 50% flowering, ND-95% = number of days to 95% maturity.
Fig. 2
Fig. 2
Dendrogram of 40 faba bean genotypes from SRAP markers by Jaccard’s coefficient and the UPGMA clustering method. Similarity values are shown at bottom of dendrogram.
Fig. 3
Fig. 3
Dendrogram of 40 faba bean genotypes from AFLP markers by Jaccard’s coefficient and the UPGMA clustering method. Similarity values are shown at bottom of dendrogram.
Fig. 4
Fig. 4
Dendrogram of 40 faba bean genotypes from combined SRAP and AFLP markers by Jaccard’s coefficient and the UPGMA clustering method. Similarity values are shown at bottom of dendrogram.

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