Neurological morphofunctional differentiation induced by REAC technology in PC12. A neuro protective model for Parkinson's disease

Sci Rep. 2015 May 15;5:10439. doi: 10.1038/srep10439.

Abstract

Research for the use of physical means, in order to induce cell differentiation for new therapeutic strategies, is one of the most interesting challenges in the field of regenerative medicine, and then in the treatment of neurodegenerative diseases, Parkinson's disease (PD) included. The aim of this work is to verify the effect of the radio electric asymmetric conveyer (REAC) technology on the PC12 rat adrenal pheochromocytoma cell line, as they display metabolic features of PD. PC12 cells were cultured with a REAC regenerative tissue optimization treatment (TO-RGN) for a period ranging between 24 and 192 hours. Gene expression analysis of specific neurogenic genes, as neurogenin-1, beta3-tubulin and Nerve growth factor, together with the immunostaining analysis of the specific neuronal protein beta3-tubulin and tyrosine hydroxylase, shows that the number of cells committed toward the neurogenic phenotype was significantly higher in REAC treated cultures, as compared to control untreated cells. Moreover, MTT and Trypan blue proliferation assays highlighted that cell proliferation was significantly reduced in REAC TO-RGN treated cells. These results open new perspectives in neurodegenerative diseases treatment, particularly in PD. Further studies will be needed to better address the therapeutic potential of the REAC technology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Basic Helix-Loop-Helix Transcription Factors / biosynthesis
  • Basic Helix-Loop-Helix Transcription Factors / genetics
  • Cell Differentiation
  • Cell Line, Tumor
  • Cell Proliferation
  • Deep Brain Stimulation / methods*
  • Dopaminergic Neurons / cytology*
  • Electric Stimulation Therapy / methods*
  • Gene Expression Profiling
  • Nerve Growth Factor / biosynthesis
  • Nerve Growth Factor / genetics
  • Nerve Tissue Proteins / biosynthesis
  • Nerve Tissue Proteins / genetics
  • Neuroprotection / physiology*
  • PC12 Cells
  • Parkinson Disease / therapy*
  • Rats
  • Tubulin / biosynthesis
  • Tubulin / genetics
  • Tyrosine 3-Monooxygenase / metabolism

Substances

  • Basic Helix-Loop-Helix Transcription Factors
  • Nerve Tissue Proteins
  • Tubulin
  • neurogenin-1, rat
  • Nerve Growth Factor
  • Tyrosine 3-Monooxygenase