Content evaluation of 4 furanocoumarin monomers in various citrus germplasms

Food Chem. 2015 Nov 15;187:75-81. doi: 10.1016/j.foodchem.2015.04.007. Epub 2015 Apr 8.


Due to the furanocoumarin compounds in the fruit, the production and consumption of grapefruit have been affected in the past decades since the 'grapefruit juice effect' was declared. To provide elite germplasm and obtain knowledge for future citrus breeding programs, the contents of 4 furanocoumarin monomers (FCMs) in the juice sacs from 73 citrus germplasms were evaluated using ultra-performance liquid chromatography. 6',7'-Dihydroxybergamottin and bergamottin were dominant in all the tested grapefruits, while there were some pomelos with dominant epoxybergamottin, and some with dominant 6',7'-dihydroxybergamottin and bergamottin. The contents of FCMs were low or below detection in sweet oranges, mandarins, lemons and trifoliate oranges. The results also show that the dominant patterns of FCMs are genotype-related, and crossing and selection are effective approaches to alter FCM profiles in citrus breeding. Furthermore, the contribution of pomelo as a parent to grapefruit regarding their FCM profiles was discussed.

Keywords: Citrus germplasms; Furanocoumarin monomers (FCMs); Juice sacs; Ultra-performance liquid chromatography (UPLC).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid
  • Citrus / chemistry*
  • Citrus paradisi / chemistry
  • Fruit and Vegetable Juices / analysis
  • Furocoumarins / analysis*


  • Furocoumarins
  • epoxybergamottin
  • bergamottin
  • 6',7'-dihydroxybergamottin