The biased reptation model of DNA gel electrophoresis: mobility vs molecular size and gel concentration

Biopolymers. 1989 Oct;28(10):1781-91. doi: 10.1002/bip.360281011.


The biased reptation model provides a good framework for interpreting the results of continuous field DNA electrophoresis experiments performed in agarose gels. Here we discuss the main features of the mobility-molecular size and mobility-gel concentration diagrams as obtained from new extensive computer simulations of the model. Our aim is to suggest a global and coherent picture of this widely used yet poorly understood experimental technique, and to point out the areas where a systematic experimental study is still needed.

MeSH terms

  • Computer Simulation
  • DNA / analysis
  • Electrophoresis / methods*
  • Electrophoresis, Agar Gel / methods*
  • Models, Chemical
  • Molecular Weight


  • DNA