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, 2015, 461294

Chinese Medicine Bu Xu Hua Yu Recipe for the Regulation of treg/th17 Ratio Imbalance in Autoimmune Hepatitis

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Chinese Medicine Bu Xu Hua Yu Recipe for the Regulation of treg/th17 Ratio Imbalance in Autoimmune Hepatitis

Lei Wang et al. Evid Based Complement Alternat Med.

Abstract

Objectives. The aim of this study is researching the role of the Regulatory T cell (Treg)/T helper cell-17 (Th17) cell ratio imbalance in the pathogenesis of autoimmune hepatitis (AIH) and the use of the "Bu Xu Hua Yu" recipe in the treatment of AIH. Materials and Methods. Sixty adult male C57/BL6 mice were divided into six different groups. α-Galcer was injected abdominally for production of the animal models. Liver function tests, histological examinations, liver tissue Regulatory T cell, and T helper cell-17 levels tests were carried out. TGF-β1, IL-10, IL-17, and expression of mRNA and protein levels of Foxp3 and ROR-γt were also assessed. Results. Bu Xu Hua Yu method increased the levels of Regulatory T cell, IL-10, and the expression of Foxp3 (P < 0.05) in mice liver tissues. Furthermore, there were decreases in the levels of T helper cell-17, IL-17, and expression of RORγt mRNA and protein (P < 0.05). The ratio of Treg/Th17 was increased (P < 0.05). Conclusion. Mice with AIH have a Treg/Th17 ratio imbalance. Bu Xu Hua Yu method was able to restore the cellular balance of Treg/Th17 through the regulation of the expression of RORγt and Foxp3 and can play an important role in the treatment of AIH.

Figures

Figure 1
Figure 1
The figure shows the histological analysis for control group (K), model group (M), steroids group (X), and Chinese medicine high dose group (G). Histological analysis for each group.
Figure 2
Figure 2
The figure shows the level of ALT, AST, and AKP for each of the six groups.
Figure 3
Figure 3
Treg (CD4+CD25+Foxp3+) FACS hepatic tissue for each group.
Figure 4
Figure 4
Th17 (CD3+CD4+IL-17+IFN-γ−) FACS hepatic tissue for each group.
Figure 5
Figure 5
The percentage of Th17 cells and Treg cells and the ratio of the two types of cells.
Figure 6
Figure 6
Analysis of hepatic tissue cytokines.
Figure 7
Figure 7
Imaging of Foxp3, RORγt mRNA gel electrophoresis.
Figure 8
Figure 8
Foxp3, RORγt mRNA real-time PCR images for each group.
Figure 9
Figure 9
The protein expression of Foxp3 and RORγt for each hepatic tissue group.
Figure 10
Figure 10
Results for the analysis of hepatic Foxp3 and RORγt proteins.

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