Q-bank phytoplasma: a DNA barcoding tool for phytoplasma identification

Nicoletta Contaldo; Samanta Paltrinieri; Olga Makarova; Assunta Bertaccini; Mogens Nicolaisen

doi:10.1007/978-1-4939-2620-6_10

Q-bank phytoplasma: a DNA barcoding tool for phytoplasma identification

Methods Mol Biol. 2015:1302:123-35. doi: 10.1007/978-1-4939-2620-6_10.

Authors

Nicoletta Contaldo¹, Samanta Paltrinieri, Olga Makarova, Assunta Bertaccini, Mogens Nicolaisen

Affiliation

¹ DipSA, Plant Pathology, Alma Mater Studiorum-University of Bologna, VialeFanin 42, Bologna, I-40127, Italy.

PMID: 25981251
DOI: 10.1007/978-1-4939-2620-6_10

Abstract

DNA barcoding is an identification method based on comparison of a short DNA sequence with known sequences from a database. A DNA barcoding tool has been developed for phytoplasma identification. This phytoplasma DNA barcoding protocol based on the tuf gene has been shown to identify phytoplasmas belonging to the following groups: 16SrI, 16SrII, 16SrIII, 16SrIV, 16SrV, 16SrVI, 16SrVII, 16SrIX, 16SrX, 16SrXI, 16SrXII, 16SrXIV, 16SrXX, and 16SrXXI.

MeSH terms

DNA Barcoding, Taxonomic*
DNA, Bacterial / genetics*
Electrophoresis, Agar Gel
Genes, Bacterial / genetics*
Phytoplasma / classification*
Phytoplasma / genetics*
Polymerase Chain Reaction / methods*
Sequence Analysis, DNA / methods*

Substances

DNA, Bacterial