Molecular-based diagnosis of respiratory allergic diseases in children from Curitiba, a city in Southern Brazil

Allergol Immunopathol (Madr). 2016 Jan-Feb;44(1):18-22. doi: 10.1016/j.aller.2015.03.001. Epub 2015 May 13.


Background: Prevalence of respiratory allergic diseases has increased worldwide. Identification of the aeroallergens involved in allergenic sensitisation is important for diagnosis, treatment and prevention.

Objective: To verify the molecular pattern of sensitisation to aeroallergens in patients with allergic respiratory diseases using microarray technique for specific IgE antibody detection.

Methods: Cross-sectional study of 101 children with allergic rhinitis was followed in an outpatient clinic. All patients had positive skin prick tests (SPT) to at least one of the following antigens: Dermatophagoides pteronyssinus, Blomia tropicalis, Blattella germanica, Lolium multiflorum, and dog and cat epithelium. Serum specific IgE antibodies (sIgE) to mites, animal epithelia, fungi, cockroach and pollens components were determined by ImmunoCAP ISAC.

Results: sIgE to group 1 and 2 mite allergens showed higher positive rates: Der p 1 (74.2%), Der p 2 (73.3%), Der f 1 (74.2%), Der f 2 (72.3%). sIgE to animal epithelia were less frequent, Can f 1, Can f 2, Can f 3 in 4.9%, 2.9%, 1.9% respectively and Fel d 1, Fel d 2, Fel d 4 in 16.8%, 0.9% and 1.9%. respectively. Sensitisation to fungi and cockroach were rare, except for Bla g 7, to which 16.8% were positive. There was no significant recognition for tree pollens group. For grass, sIgE were detected to Cyn d 1 in 16.8%, Phl p 1 and Phl p 4 in 14.8% and 12.9%, respectively.

Conclusion: Knowing that the pattern of allergic sensitisation varies according to environment and population, our results reinforce the need for local studies, using molecular-based diagnosis.

Keywords: Allergens; Asthma; Children; Molecular diagnostic testing; Rhinitis.

MeSH terms

  • Adolescent
  • Air Pollutants / immunology
  • Air Pollutants / metabolism*
  • Allergens / immunology
  • Allergens / metabolism*
  • Animals
  • Antigens, Dermatophagoides / immunology
  • Arthropod Proteins / immunology
  • Brazil
  • Child
  • Cross-Sectional Studies
  • Cysteine Endopeptidases / immunology
  • Humans
  • Immunoglobulin E / blood
  • Immunologic Tests
  • Microarray Analysis
  • Pathology, Molecular
  • Pyroglyphidae / immunology
  • Rhinitis, Allergic / diagnosis*
  • Rhinitis, Allergic / immunology


  • Air Pollutants
  • Allergens
  • Antigens, Dermatophagoides
  • Arthropod Proteins
  • Immunoglobulin E
  • Cysteine Endopeptidases
  • Dermatophagoides farinae antigen f 1