Down-regulation of a serine protease, myeloblastin, causes growth arrest and differentiation of promyelocytic leukemia cells

Cell. 1989 Dec 22;59(6):959-68. doi: 10.1016/0092-8674(89)90752-6.


Cells from the human leukemia cell line HL-60 undergo terminal differentiation when exposed to inducing agents. Differentiation of these cells is always accompanied by withdrawal from the cell cycle. Here we describe the isolation of a cDNA encoding a novel serine protease that is present in HL-60 cells and is down-regulated during induced differentiation of these cells. We have named this protease myeloblastin. Down-regulation of myeloblastin mRNA occurs with both monocytic and granulocytic inducers. Myeloblastin mRNA is undetectable in fully differentiated HL-60 cells as well as in human peripheral blood monocytes. We found that regulation of myeloblastin mRNA in HL-60 cells is serum dependent. Inhibition of myeloblastin expression by an antisense oligodeoxynucleotide inhibits proliferation and induces differentiation of promyelocyte-like leukemia cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cell Differentiation / drug effects
  • Cell Division / drug effects
  • Cell Line
  • Culture Media
  • DNA, Neoplasm / genetics
  • Gene Expression Regulation, Enzymologic*
  • Genes*
  • Humans
  • Leukemia, Promyelocytic, Acute
  • Molecular Sequence Data
  • Myeloblastin
  • Oligodeoxyribonucleotides / pharmacology
  • RNA, Messenger / genetics
  • Serine Endopeptidases / biosynthesis
  • Serine Endopeptidases / genetics*
  • Transcription, Genetic
  • Tumor Cells, Cultured / cytology
  • Tumor Cells, Cultured / drug effects
  • Tumor Cells, Cultured / enzymology*


  • Culture Media
  • DNA, Neoplasm
  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • Serine Endopeptidases
  • Myeloblastin

Associated data

  • GENBANK/M29142