Immunoreactive anionic and cationic trypsin in human serum

Clin Chim Acta. 1989 Sep 15;184(1):31-46. doi: 10.1016/0009-8981(89)90254-4.

Abstract

A simple method for the purification of anionic and cationic trypsinogen and trypsin from human pancreatic juice applying affinity chromatography on aprotinin coupled Sepharose is described together with the N-terminal amino acid sequences for both trypsinogens. In addition, enzyme-linked immunoabsorbent assay (ELISA) methods for the determination of anionic and cationic trypsin-like immunoreactivity (irAT and irCT) are described. Normal serum levels are 21.3 +/- 7.4 micrograms/l and 27.8 +/- 9.0 microgram/l for irAT and irCT respectively and the accuracy of these assays is 6-10%. In our population, the normal ratio between irCT and irAT in serum is 1.36 +/- 0.42. In normal serum trypsin-like immunoreactivity consists solely of trypsinogen. In acute pancreatitis there is an increase over normal of both irAT and irCT with a proportionally greater increase in irAT than irCT. Similar changes are also found in uremic patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Amino Acid Sequence
  • Anions
  • Cations
  • Chromatography, Affinity
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Kidney Diseases / enzymology
  • Molecular Sequence Data
  • Molecular Weight
  • Pancreatic Juice / enzymology
  • Pancreatic Neoplasms / enzymology
  • Pancreatitis / enzymology
  • Trypsin / blood*
  • Trypsin / isolation & purification
  • Trypsin Inhibitors / metabolism
  • Trypsinogen / blood*
  • Trypsinogen / isolation & purification

Substances

  • Anions
  • Cations
  • Trypsin Inhibitors
  • Trypsinogen
  • Trypsin