In vivo single-cell labeling by confined primed conversion

Nat Methods. 2015 Jul;12(7):645-8. doi: 10.1038/nmeth.3405. Epub 2015 May 18.


Spatially confined green-to-red photoconversion of fluorescent proteins with high-power, pulsed laser illumination is negligible, thus precluding optical selection of single cells in vivo. We report primed conversion, in which low-power, dual-wavelength, continuous-wave illumination results in pronounced photoconversion. With a straightforward addition to a conventional confocal microscope, we show confined primed conversion in living zebrafish and reveal the complex anatomy of individual neurons packed between neighboring cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Microscopy, Fluorescence, Multiphoton / methods*
  • Neurons / cytology*
  • Zebrafish