Effect of sesamin on apoptosis and cell cycle arrest in human breast cancer mcf-7 cells

Asian Pac J Cancer Prev. 2015;16(9):3779-83. doi: 10.7314/apjcp.2015.16.9.3779.

Abstract

Dietary prevention has been known to reduce breast cancer risk. Sesamin is one of the major components in sesame seeds and has been widely studied and proven to have anti-proliferation and anti-angiogenic effects on cancer cells. In this study, the influence of sesamin was tested in the human breast cancer MCF-7 cell line for cell viability (MTT assay) and cell cycling (flow cytometry). Results showed that sesamin dose-dependently (1, 10 and 50 μM) reduced the cell viability and increased LDH release and apoptosis (TUNEL assay). In addition, there was a significant increase of sub-G1 phase arrest in the cell cycle after sesamin treatment. Furthermore, sesamin increased the expression of apoptotic markers of Bax, caspase-3, and cell cycle control proteins, p53 and checkpoint kinase 2. Taken together, these results suggested that sesamin might be used as a dietary supplement for prevention of breast cancer by modulating apoptotic signal pathways and inhibiting tumor cell growth.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / pharmacology*
  • Apoptosis / drug effects*
  • Blotting, Western
  • Breast Neoplasms / drug therapy*
  • Breast Neoplasms / metabolism
  • Breast Neoplasms / pathology*
  • Cell Cycle Checkpoints / drug effects*
  • Cell Proliferation / drug effects*
  • Checkpoint Kinase 2 / metabolism
  • Dioxoles / pharmacology*
  • Female
  • Humans
  • Lignans / pharmacology*
  • Tumor Cells, Cultured

Substances

  • Antioxidants
  • Dioxoles
  • Lignans
  • Checkpoint Kinase 2
  • CHEK2 protein, human
  • sesamin