Identification of a novel MICB allele, MICB*030, by cloning and sequencing

Abstract

A novel MICB allele, MICB*030, has been identified in a healthy Chinese individual of Mongol ethnicity residing in northern China by polymerase chain reaction sequence-based typing (PCR-SBT) and confirmed by cloning and sequencing. MICB*030 was linked to HLA-B*35. Aligned with MICB*005:02, MICB*030 has a nonsynonymous adenine substitution at nucleotide position 50 in exon 3, leading to amino acid change from serine to arginine at codon 102 of the mature MICB molecule.