Identification of a Dual Inhibitor of SRPK1 and CK2 That Attenuates Pathological Angiogenesis of Macular Degeneration in Mice

Mol Pharmacol. 2015 Aug;88(2):316-25. doi: 10.1124/mol.114.097345. Epub 2015 May 20.

Abstract

Excessive angiogenesis contributes to numerous diseases, including cancer and blinding retinopathy. Antibodies against vascular endothelial growth factor (VEGF) have been approved and are widely used in clinical treatment. Our previous studies using SRPIN340, a small molecule inhibitor of SRPK1 (serine-arginine protein kinase 1), demonstrated that SRPK1 is a potential target for the development of antiangiogenic drugs. In this study, we solved the structure of SRPK1 bound to SRPIN340 by X-ray crystallography. Using pharmacophore docking models followed by in vitro kinase assays, we screened a large-scale chemical library, and thus identified a new inhibitor of SRPK1. This inhibitor, SRPIN803, prevented VEGF production more effectively than SRPIN340 owing to the dual inhibition of SRPK1 and CK2 (casein kinase 2). In a mouse model of age-related macular degeneration, topical administration of eye ointment containing SRPIN803 significantly inhibited choroidal neovascularization, suggesting a clinical potential of SRPIN803 as a topical ointment for ocular neovascularization. Thus SRPIN803 merits further investigation as a novel inhibitor of VEGF.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Topical
  • Animals
  • Casein Kinase II / antagonists & inhibitors*
  • Cell Line
  • Choroidal Neovascularization / drug therapy*
  • Crystallography, X-Ray
  • Disease Models, Animal
  • Enzyme Inhibitors / administration & dosage*
  • Enzyme Inhibitors / chemistry
  • Enzyme Inhibitors / pharmacology
  • Humans
  • Macular Degeneration / drug therapy
  • Macular Degeneration / pathology
  • Mice
  • Models, Molecular
  • Molecular Docking Simulation
  • Niacinamide / analogs & derivatives
  • Niacinamide / chemistry
  • Piperidines / chemistry
  • Protein-Serine-Threonine Kinases / antagonists & inhibitors*
  • Protein-Serine-Threonine Kinases / chemistry*
  • Protein-Serine-Threonine Kinases / metabolism
  • Pyrimidinones / administration & dosage*
  • Pyrimidinones / chemistry
  • Pyrimidinones / pharmacology
  • Small Molecule Libraries / administration & dosage*
  • Small Molecule Libraries / chemistry
  • Small Molecule Libraries / pharmacology
  • Structure-Activity Relationship
  • Thiadiazoles / administration & dosage*
  • Thiadiazoles / chemistry
  • Thiadiazoles / pharmacology

Substances

  • Enzyme Inhibitors
  • Piperidines
  • Pyrimidinones
  • SRPIN340
  • SRPIN803
  • Small Molecule Libraries
  • Thiadiazoles
  • Niacinamide
  • SRPK1 protein, human
  • Casein Kinase II
  • Protein-Serine-Threonine Kinases