Multitarget PCR Assay for Direct Detection of Penicillinase-Producing Neisseria gonorrhoeae for Enhanced Surveillance of Gonococcal Antimicrobial Resistance

J Clin Microbiol. 2015 Aug;53(8):2706-8. doi: 10.1128/JCM.00540-15. Epub 2015 May 20.


A multitarget PCR was developed for the direct detection of penicillinase-producing Neisseria gonorrhoeae (PPNG). The assay was validated by testing 342 PPNG isolates and 415 clinical samples. The method is suitable for routine detection of PPNG strains. Its multitarget approach reduces the potential for false-negative results caused by sequence variations.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Drug Resistance, Bacterial*
  • Epidemiological Monitoring
  • Gonorrhea / epidemiology
  • Gonorrhea / microbiology*
  • Humans
  • Neisseria gonorrhoeae / drug effects
  • Neisseria gonorrhoeae / enzymology*
  • Neisseria gonorrhoeae / genetics
  • Penicillinase / genetics*
  • Polymerase Chain Reaction / methods*


  • Penicillinase