Elastin hydrolysate derived from fish enhances proliferation of human skin fibroblasts and elastin synthesis in human skin fibroblasts and improves the skin conditions

J Sci Food Agric. 2016 Mar 30;96(5):1672-7. doi: 10.1002/jsfa.7270. Epub 2015 Jun 15.


Background: Recent studies have shown that certain peptides significantly improve skin conditions, such as skin elasticity and the moisture content of the skin of healthy woman. This study aimed to investigate the effects of elastin hydrolysate on human skin. Proliferation and elastin synthesis were evaluated in human skin fibroblasts exposed to elastin hydrolysate and proryl-glycine (Pro-Gly), which is present in human blood after elastin hydrolysate ingestion. We also performed an ingestion test with elastin hydrolysate in humans and evaluated skin condition.

Results: Elastin hydrolysate and Pro-Gly enhanced the proliferation of fibroblasts and elastin synthesis. Maximal proliferation response was observed at 25 ng mL(-1) Pro-Gly. Ingestion of elastin hydrolysate improved skin condition, such as elasticity, number of wrinkles, and blood flow. Elasticity improved by 4% in the elastin hydrolysate group compared with 2% in the placebo group.

Conclusion: Therefore, elastin hydrolysate activates human skin fibroblasts and has beneficial effects on skin conditions.

Keywords: elastin; elastin hydrolysate; fibroblasts; skin.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Administration, Topical
  • Adult
  • Animals
  • Cell Proliferation
  • Cells, Cultured
  • Double-Blind Method
  • Elastin / chemistry*
  • Elastin / metabolism
  • Elastin / pharmacology*
  • Female
  • Fibroblasts / drug effects*
  • Fibroblasts / physiology
  • Fishes
  • Humans
  • Hydrolysis
  • Skin / cytology*
  • Skin / drug effects*


  • Elastin