Human chorionic gonadotropin stimulates spheroid attachment on fallopian tube epithelial cells through the mitogen-activated protein kinase pathway and down-regulation of olfactomedin-1

Fertil Steril. 2015 Aug;104(2):474-82. doi: 10.1016/j.fertnstert.2015.04.030. Epub 2015 May 18.


Objective: To study the effect of human chorionic gonadotropin (hCG) on olfactomedin-1 (Olfm1) expression and spheroid attachment in human fallopian tube epithelial cells in vitro.

Design: Experimental study.

Setting: Reproductive biology laboratory.

Patient(s): Healthy nonpregnant women.

Intervention(s): No patient interventions.

Main outcome measure(s): Luteinizing hormone/chorionic gonadotropin receptor (LHCGR) and Olfm1 expression in fallopian tube epithelium cell line (OE-E6/E7 cells). OE-E6/E7 cells treated with hCG, U0126 extracellular signal-regulated kinase (ERK) inhibitor, or XAV939 Wnt/β-catenin inhibitor were analyzed by Western blotting, real-time polymerase chain reaction, and in vitro spheroid attachment assay.

Result(s): Human chorionic gonadotropin increased spheroid attachment on OE-E6/E7 cells through down-regulation of Olfm1 and activation of Wnt and mitogen-activated protein kinase (MAPK) signaling pathways. U0126 down-regulated both MAPK and Wnt/β-catenin signaling pathways and up-regulated Olfm1 expression. XAV939 down-regulated only the Wnt/β-catenin signaling pathway but up-regulated Olfm1 expression.

Conclusion(s): Human chorionic gonadotropin activated both ERK and Wnt/β-catenin signaling pathways and enhanced spheroid attachment on fallopian tube epithelial cells through down-regulation of Olfm1 expression.

Keywords: MAPK signaling; Olfactomedin-1; Wnt-signaling; fallopian tube; tubal ectopic pregnancy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Cell Line
  • Chorionic Gonadotropin / pharmacology*
  • Coculture Techniques
  • Dose-Response Relationship, Drug
  • Down-Regulation / drug effects
  • Down-Regulation / physiology
  • Epithelial Cells / drug effects
  • Epithelial Cells / metabolism*
  • Extracellular Matrix Proteins / metabolism*
  • Fallopian Tubes / cytology
  • Fallopian Tubes / drug effects
  • Fallopian Tubes / metabolism*
  • Female
  • Glycoproteins / metabolism*
  • Humans
  • MAP Kinase Signaling System / drug effects
  • MAP Kinase Signaling System / physiology*
  • Middle Aged
  • Trophoblasts / metabolism*


  • Chorionic Gonadotropin
  • Extracellular Matrix Proteins
  • Glycoproteins
  • olfactomedin