Insulin-like growth factor 1 induction of differentiation of porcine preadipocytes

J Anim Sci. 1989 Sep;67(9):2452-9. doi: 10.2527/jas1989.6792452x.


The role of insulin-like growth factor I (IGF-1) in the development of the porcine preadipocyte was studied. Primary cultures of stromal-vascular cells (containing preadipocytes) were derived from s.c. adipose tissue of pigs at 1 d of age by enzyme digestion and centrifugation. Cells were cultured for a total of 15 d. Cells were exposed to IGF-1 at concentrations of 0, 5, 25 or 50 ng/ml medium during one of four time periods: d 1-15, d 1-5, d 13-15, or 4 h on d 15 of culture. IGF-1 had a mitogenic effect on cells during the first three time periods as determined by coulter counting. IGF-1 induced the enzymatic differentiation of porcine preadipocytes following exposure for either the entire 15 d of culture or for only 48 h (d 13-15) after confluency had been attained (d 5). Histochemically, lipid accumulation over time paralleled changes in enzyme activity. Incubation of IGF-1 with the cell cultures during the logarithmic phase of growth (d 1-5) or for 4 h on d 15 did not affect enzyme activity. These data indicate that IGF-1 can induce the differentiation of porcine preadipocytes after the cells leave the logarithmic phase of growth through action on post-confluent events.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adipose Tissue / cytology*
  • Adipose Tissue / drug effects
  • Adipose Tissue / enzymology
  • Animals
  • Cell Count / veterinary
  • Cell Differentiation / drug effects
  • Cell Division
  • Cells, Cultured
  • Histocytochemistry
  • Insulin-Like Growth Factor I / pharmacology*
  • Lipid Metabolism
  • Male
  • Somatomedins / pharmacology*


  • Somatomedins
  • Insulin-Like Growth Factor I