Expanding the Biologist's Toolkit With CRISPR-Cas9

Mol Cell. 2015 May 21;58(4):568-74. doi: 10.1016/j.molcel.2015.02.032.

Abstract

Few discoveries transform a discipline overnight, but biologists today can manipulate cells in ways never possible before, thanks to a peculiar form of prokaryotic adaptive immunity mediated by clustered regularly interspaced short palindromic repeats (CRISPR). From elegant studies that deciphered how these immune systems function in bacteria, researchers quickly uncovered the technological potential of Cas9, an RNA-guided DNA cleaving enzyme, for genome engineering. Here we highlight the recent explosion in visionary applications of CRISPR-Cas9 that promises to usher in a new era of biological understanding and control.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • CRISPR-Associated Proteins / genetics
  • CRISPR-Associated Proteins / metabolism*
  • CRISPR-Cas Systems / genetics
  • Clustered Regularly Interspaced Short Palindromic Repeats / genetics*
  • Deoxyribonuclease I / genetics
  • Deoxyribonuclease I / metabolism*
  • Genetic Engineering / methods*
  • Genome / genetics
  • Humans
  • Models, Genetic
  • RNA, Guide / genetics
  • RNA, Guide / metabolism*

Substances

  • CRISPR-Associated Proteins
  • RNA, Guide
  • Deoxyribonuclease I