TNF compromises lysosome acidification and reduces α-synuclein degradation via autophagy in dopaminergic cells

Exp Neurol. 2015 Sep;271:112-21. doi: 10.1016/j.expneurol.2015.05.008. Epub 2015 May 19.

Abstract

Tumor necrosis factor-α (TNF) is increasingly implicated as a critical pro-inflammatory cytokine involved in chronic inflammation and neurodegeneration of Parkinson's disease (PD). However, the cellular and molecular events that lead to dopaminergic neuron degeneration are not fully understood. In this study, we demonstrated that microglia-released and recombinant TNF disrupted α-synuclein (α-SYN) degradation and caused its accumulation in PC12 cells and midbrain neurons. At subtoxic doses, recombinant TNF was found to increase the number of LC3 puncta dots and LC3II protein level, associated with the increases of P62 protein level. Inhibition of lysosomal degradation with Bafilomycin A1 pretreatment abrogated the TNF-induced elevation in LC3II protein level whereas autophagy inhibitor 3-methyladenine did not affect it. Moreover, TNF led to a marked increase in the number of yellow LC3 dots with a marginal elevation in red-only dots in RFP-GFP-tandem fluorescent LC3 (tf-LC3) transfected PC12 cells, implying the impairment in autophagic flux. Furthermore, TNF treatment reduced lysosomal acidification, as LysoTracker Red fluorescence and LysoSensor fluorescence shift from blue to yellow was markedly decreased in TNF-treated PC12 cells. Co-treatment with mammalian target of rapamycin kinase complex 1 (mTORC1) inhibitor PP242, which activated transcription factor EB (TFEB) signaling and lysosome biogenesis, partially rescued the accumulation of α-SYN in PC12 cells and midbrain neurons. Taken together, our results demonstrated that at subtoxic levels, TNF was able to impair autophagic flux and result in α-SYN accumulation by compromising lysosomal acidification in dopaminergic cells. This may represent a novel mechanism for TNF-induced dopaminergic neuron degeneration in PD.

Keywords: Autophagy; Dopaminergic cells; Lysosome acidification; TNF; α-Synuclein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autophagy / drug effects
  • Autophagy / physiology*
  • Cell Survival
  • Cells, Cultured
  • Dopaminergic Neurons / drug effects
  • Dopaminergic Neurons / metabolism*
  • Embryo, Mammalian
  • Female
  • Hydrogen-Ion Concentration
  • Indoles / pharmacology
  • Interleukin-1beta / genetics
  • Interleukin-1beta / metabolism
  • Lipopolysaccharides / pharmacology
  • Lysosomal-Associated Membrane Protein 1 / metabolism
  • Lysosomes / drug effects
  • Lysosomes / physiology*
  • Mesencephalon / cytology
  • PC12 Cells
  • Pregnancy
  • Purines / pharmacology
  • Rats
  • Rats, Sprague-Dawley
  • Sincalide / metabolism
  • Tumor Necrosis Factor-alpha / metabolism*
  • Tumor Necrosis Factor-alpha / pharmacology
  • alpha-Synuclein / genetics
  • alpha-Synuclein / metabolism*

Substances

  • Indoles
  • Interleukin-1beta
  • Lipopolysaccharides
  • Lysosomal-Associated Membrane Protein 1
  • Purines
  • Tumor Necrosis Factor-alpha
  • alpha-Synuclein
  • PP242
  • Sincalide