Differential expression of microRNAs in renal transplant patients with acute T-cell mediated rejection

Transpl Immunol. 2015 Sep;33(1):1-6. doi: 10.1016/j.trim.2015.05.002. Epub 2015 May 20.


Background: MicroRNAs (miRNAs) regulate most of encoding genes and protein. In this study, we aimed to investigate the expression levels of miR-142-5p, miR-142-3p, miR-155 and miR-223 in paired biopsy and peripheral blood mononuclear cell (PBMC) samples of renal allograft recipients with acute T-cell mediated rejection (ATCMR), compared with normal allografts (NA).

Methods: In this study, the expression levels of individual miRNAs were determined in biopsy and PBMC samples of 17 recipients with ATCMR and 18 recipients with NA.

Results: Our results showed that the intragraft expression levels of all studied miRNAs were significantly higher in ATCMR than NA. However, regarding the PBMC samples, miR-142-3p and miR-223 were significantly increased in ATCMR than NA. Receiver operating characteristic (ROC) analysis showed that miR-142-5p, miR-142-3p, miR-155 and miR-223 in biopsy samples and miR-142-3p and miR-223 in PBMC samples could discriminate ATCMR from NA recipients.

Conclusion: It has been reported that high intragraft expressions of miRNAs have a profound role in the pathogenesis of ATCMR process. Our results showed that high expression of all the studied miRNAs in biopsies and miR-142-3p and miR-223 in PBMC samples could be used as suggestive diagnostic tools to discriminate ATCMR patients from NA.

Keywords: Acute T-cell mediated rejection; Biomarker; MicroRNA; Renal transplantation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Adult
  • Aged
  • Biopsy
  • Female
  • Gene Expression Regulation / immunology*
  • Graft Rejection / diagnosis
  • Graft Rejection / immunology*
  • Graft Rejection / pathology
  • Humans
  • Kidney / immunology*
  • Kidney / pathology
  • Kidney Transplantation*
  • Male
  • MicroRNAs / immunology*
  • Middle Aged
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / pathology


  • MIRN142 microRNA, human
  • MIRN223 microRNA, human
  • MicroRNAs