Mitotic Control of Planar Cell Polarity by Polo-like Kinase 1

Dev Cell. 2015 Jun 8;33(5):522-34. doi: 10.1016/j.devcel.2015.03.024. Epub 2015 May 21.

Abstract

During cell division, polarized epithelial cells employ mechanisms to preserve cell polarity and tissue integrity. In dividing cells of the mammalian skin, planar cell polarity (PCP) is maintained through the bulk internalization, equal segregation, and polarized recycling of cortical PCP proteins. The dramatic redistribution of PCP proteins coincides precisely with cell-cycle progression, but the mechanisms coordinating PCP and mitosis are unknown. Here we identify Plk1 as a master regulator of PCP dynamics during mitosis. Plk1 interacts with core PCP component Celsr1 via a conserved polo-box domain (PBD)-binding motif, localizes to mitotic endosomes, and directly phosphorylates Celsr1. Plk1-dependent phosphorylation activates the endocytic motif specifically during mitosis, allowing bulk recruitment of Celsr1 into endosomes. Inhibiting Plk1 activity blocks PCP internalization and perturbs PCP asymmetry. Mimicking dileucine motif phosphorylation is sufficient to drive Celsr1 internalization during interphase. Thus, Plk1-mediated phosphorylation of Celsr1 ensures that PCP redistribution is precisely coordinated with mitotic entry.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acid Motifs
  • Amino Acid Sequence
  • Animals
  • Cell Cycle Proteins / metabolism*
  • Cell Polarity / physiology*
  • Endocytosis / physiology
  • Endosomes / metabolism*
  • Fluorescent Antibody Technique
  • HeLa Cells
  • Humans
  • Interphase
  • Keratinocytes / cytology
  • Keratinocytes / metabolism*
  • Mice
  • Mitosis / physiology*
  • Molecular Sequence Data
  • Phosphorylation
  • Protein Binding
  • Protein-Serine-Threonine Kinases / metabolism*
  • Proto-Oncogene Proteins / metabolism*
  • Receptors, G-Protein-Coupled / metabolism*
  • Sequence Homology, Amino Acid
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Cell Cycle Proteins
  • Celsr1 protein, mouse
  • Proto-Oncogene Proteins
  • Receptors, G-Protein-Coupled
  • Protein-Serine-Threonine Kinases
  • polo-like kinase 1