Haemocytes Control Stem Cell Activity in the Drosophila Intestine

Nat Cell Biol. 2015 Jun;17(6):736-48. doi: 10.1038/ncb3174. Epub 2015 May 25.

Abstract

Coordination of stem cell activity with inflammatory responses is critical for regeneration and homeostasis of barrier epithelia. The temporal sequence of cell interactions during injury-induced regeneration is only beginning to be understood. Here we show that intestinal stem cells (ISCs) are regulated by macrophage-like haemocytes during the early phase of regenerative responses of the Drosophila intestinal epithelium. On tissue damage, haemocytes are recruited to the intestine and secrete the BMP homologue DPP, inducing ISC proliferation by activating the type I receptor Saxophone and the Smad homologue SMOX. Activated ISCs then switch their response to DPP by inducing expression of Thickveins, a second type I receptor that has previously been shown to re-establish ISC quiescence by activating MAD. The interaction between haemocytes and ISCs promotes infection resistance, but also contributes to the development of intestinal dysplasia in ageing flies. We propose that similar interactions influence pathologies such as inflammatory bowel disease and colorectal cancer in humans.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Active Transport, Cell Nucleus
  • Aging / immunology
  • Animals
  • Animals, Genetically Modified
  • Cell Proliferation
  • Cells, Cultured
  • Colorectal Neoplasms / metabolism
  • Colorectal Neoplasms / pathology
  • Drosophila Proteins / biosynthesis
  • Drosophila Proteins / metabolism
  • Drosophila melanogaster / cytology*
  • Drosophila melanogaster / genetics
  • Drosophila melanogaster / growth & development
  • Enzyme Activation / immunology
  • ErbB Receptors / metabolism
  • Hemocytes / cytology*
  • Hemocytes / metabolism
  • Hemocytes / transplantation
  • Immune Tolerance / immunology
  • Inflammation / immunology
  • Inflammatory Bowel Diseases / metabolism
  • Inflammatory Bowel Diseases / pathology
  • Intestinal Mucosa / cytology*
  • Intestinal Mucosa / immunology
  • Intestinal Mucosa / pathology
  • Janus Kinases / metabolism
  • Life Expectancy
  • Macrophages / immunology
  • Protein-Serine-Threonine Kinases / biosynthesis
  • Receptors, Cell Surface / biosynthesis
  • Receptors, Invertebrate Peptide / metabolism
  • Receptors, Transforming Growth Factor beta / metabolism
  • Regeneration / immunology
  • Smad Proteins, Receptor-Regulated / metabolism
  • Stem Cells / cytology*
  • Stem Cells / immunology
  • Stem Cells / metabolism
  • Tight Junctions / immunology*

Substances

  • Drosophila Proteins
  • Receptors, Cell Surface
  • Receptors, Invertebrate Peptide
  • Receptors, Transforming Growth Factor beta
  • Smad Proteins, Receptor-Regulated
  • Smox protein, Drosophila
  • dpp protein, Drosophila
  • sax protein, Drosophila
  • tkv protein, Drosophila
  • Egfr protein, Drosophila
  • ErbB Receptors
  • Janus Kinases
  • Protein-Serine-Threonine Kinases