Detection of Chlamydia trachomatis, Ureaplasma urealyticum and Mycoplasma hominis in infertile Bulgarian men with multiplex real-time polymerase chain reaction

APMIS. 2015 Jul;123(7):586-8. doi: 10.1111/apm.12391. Epub 2015 May 22.

Abstract

The effect of Chlamydia trachomatis, Ureaplasma urealyticum and Mycoplasma hominis over the sperm quality is still unclear. The aim of this study was to determine their prevalence in infertile Bulgarian men. A total of 281 men were examined by applying mRT-PCR. The registered prevalence was as follows: C. trachomatis - 13.9%, U. urealyticum - 19.2%, M. hominis - 9.9%. Co-infection was established in eight swabs. This first in Bulgaria to study for detection of chlamydia and mycoplasmas in infertile men by mRT-PCR demonstrates higher prevalence of the tested microorganisms in the infertile group toward the control one.

Keywords: Chlamydia; Mycoplasmas; infertile men; mRT-PCR; prevalence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bulgaria
  • Case-Control Studies
  • Chlamydia trachomatis / isolation & purification*
  • Coinfection
  • DNA, Bacterial / isolation & purification
  • Humans
  • Infertility, Male / microbiology*
  • Male
  • Mycoplasma hominis / isolation & purification*
  • Real-Time Polymerase Chain Reaction / methods*
  • Ureaplasma Infections / diagnosis*
  • Ureaplasma urealyticum / isolation & purification*
  • Urethra / microbiology

Substances

  • DNA, Bacterial