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. 2014 Sep 3;28(5):941-948.
doi: 10.1080/13102818.2014.948257. Epub 2014 Oct 30.

Gene Expression of Enzymes Involved in Utilization of Xylooligosaccharides by Lactobacillus Strains

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Free PMC article

Gene Expression of Enzymes Involved in Utilization of Xylooligosaccharides by Lactobacillus Strains

Ananieva Maria et al. Biotechnol Biotechnol Equip. .
Free PMC article

Abstract

Prebiotics are defined as food components that confer health benefits on the host through modulation of the microbiota. Xylooligosaccharides (XOS) are non-digestible oligosaccharides that have recently received increasing attention as potential prebiotic candidates. XOS are sugar oligomers composed of 1,4-linked xylopyranosyl backbone and are obtained by either chemical or, more commonly, enzymatic hydrolysis of xylan polysaccharides, extracted from the plant cell wall. The bifidogenic effect of XOS was demonstrated by both in vitro studies and small-scale in vivo human studies. Some intestinal bacterial strains are able to grow on XOS, yet numerous studies have demonstrated that the ability to utilize these oligosaccharides varies considerably among these bacteria. The aim of this study is to investigate the ability of several strains Lactobacillus to use XOS. Fifteen Lactobacillus strains, allifiated to L. plantarum, L. brevis and L. sakei, were studied. Screening procedure was performed for the ability of the strains to utilize XOS as an alternative carbon source. Only some of them utilize XOS. The growth kinetics show the presence of two lag phases, indicating that these bacteria utilize probably some monosaccharides present in the used XOS. XOS were fermented with high specificity by Bifidobacteria strains, but Lactobacilli did not metabolize XOS efficiently.

Keywords: Lactobacillus; XOS; gene expression; xylooligosaccharides.

Figures

Figure 1.
Figure 1.
Gene expression levels of xylanase, β-xylosidase and glucose-6-phosphate dehydrogenase in Lactobacillus plantarum.
Figure 2.
Figure 2.
Gene expression levels of β-xylosidase and glucose-6-phosphate dehydrogenase in Lactobacillus plantarum.
Figure 3.
Figure 3.
Gene expression levels of xylanase, β-xylosidase and glucose-6-phosphate dehydrogenase in Lactobacillus brevis.
Figure 4.
Figure 4.
Gene expression levels of xylanase, β-xylosidase and glucose-6-phosphate dehydrogenase in Lactobacillus sakei.
Figure 5.
Figure 5.
RT-Minus control and no-template control (NTC).
Figure 6.
Figure 6.
Antimicrobial activity of L. plantarum and L. brevis (2) against E. coli 3398 on 24th and 48th hour 1 – L. plantarum 2 – L. brevis.
Figure 7.
Figure 7.
Antimicrobial activity of L. plantarum (1) and L. brevis (2) against Enterobacter 3690 on 24th and 48th hour 1 – L. plantarum 2 – L. brevis.
Figure 8.
Figure 8.
Antimicrobial activity of L. plantarum (1) and L. brevis (2) against Staphylococcus aureus 746 on 24th and 48th hour 1 – L. plantarum 2 – L. brevis.

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Grant support

This work was supported by research grant of NSF Bulgaria [grant number BG B01/7-2012].

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