Metabolic profiles in the response to supplementation with composite antimicrobial peptides in piglets challenged with deoxynivalenol

J Anim Sci. 2015 Mar;93(3):1114-23. doi: 10.2527/jas.2014-8229.


Deoxynivalenol (DON) causes various toxic effects in human and animals. However, our previous studies have shown that composite antimicrobial peptides (CAP) can have a protective effect in piglets challenged with DON. This study was conducted to evaluate the effect of the CAP GLAM 180# on the metabolism of piglets challenged with DON using a nuclear magnetic resonance (NMR)-based metabolomics approach. A total of 28 individually housed piglets (Duroc × Landrace × Large Yorkshire) weaned at 28 d of age were randomly assigned into 4 treatment groups (7 pigs/treatment) based on a 2 × 2 factorial arrangement that were fed, respectively, a basal diet (NC), basal diet + 0.4% CAP (basal + CAP), basal diet + 4 mg/kg DON (basal + DON), and basal diet + 4 mg/kg DON + 0.4% CAP (DON + CAP). A 7-d adaptation period was followed by 30 d of treatment. Blood samples were then collected for metabolite analysis by proton NMR (H-NMR) spectroscopy and liquid chromatography tandem mass spectrometry (LC-MS/MS). The combined results of H-NMR spectroscopy and LC-MS/MS showed that DON increased ( < 0.05) the serum concentrations of low-density lipoprotein, glycoprotein, urea, trimethylamine-N-oxide (TMAO), and lactate as well as those of almost all essential AA and some nonessential AA but decreased the concentrations of high-density lipoprotein (HDL), unsaturated lipids, citrate, choline, and fumarate compared with those in NC treatment ( < 0.05). There was a significant interaction effect ( < 0.05) of supplementation with DON and CAP on some metabolites showed that the serum concentrations of HDL, unsaturated lipids, Pro, citrate, and fumarate were greater ( < 0.05) whereas those of glycoprotein, urea, TMAO, Gly, and lactate were lower in the DON + CAP treatment compared with those in the basal + DON treatment ( < 0.05). These findings indicated that DON causes disturbances in AA, lipid, and energy metabolism and that CAP could partially attenuate the above metabolic disturbances induced by DON.

Publication types

  • Comparative Study
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / blood
  • Animal Feed* / analysis
  • Animals
  • Antimicrobial Cationic Peptides / administration & dosage
  • Antimicrobial Cationic Peptides / analysis
  • Antimicrobial Cationic Peptides / pharmacology*
  • Diet
  • Dietary Supplements
  • Energy Metabolism / drug effects
  • Energy Metabolism / physiology
  • Lipid Metabolism / drug effects
  • Lipid Metabolism / physiology
  • Magnetic Resonance Spectroscopy
  • Metabolome / drug effects*
  • Metabolome / physiology
  • Swine / metabolism*
  • Tandem Mass Spectrometry
  • Trichothecenes / adverse effects*
  • Trichothecenes / analysis
  • Trichothecenes / pharmacology*


  • Amino Acids
  • Antimicrobial Cationic Peptides
  • Trichothecenes
  • deoxynivalenol