Transcription factor genes essential for cell proliferation and replicative lifespan in budding yeast

Biochem Biophys Res Commun. 2015 Jul 31;463(3):351-6. doi: 10.1016/j.bbrc.2015.05.067. Epub 2015 May 28.


Many of the lifespan-related genes have been identified in eukaryotes ranging from the yeast to human. However, there is limited information available on the longevity genes that are essential for cell proliferation. Here, we investigated whether the essential genes encoding DNA-binding transcription factors modulated the replicative lifespan of Saccharomyces cerevisiae. Heterozygous diploid knockout strains for FHL1, RAP1, REB1, and MCM1 genes showed significantly short lifespan. (1)H-nuclear magnetic resonance analysis indicated a characteristic metabolic profile in the Δfhl1/FHL1 mutant. These results strongly suggest that FHL1 regulates the transcription of lifespan related metabolic genes. Thus, heterozygous knockout strains could be the potential materials for discovering further novel lifespan genes.

Keywords: Essential gene; Heterozygous knockout; Metabolome; Replicative lifespan; Transcription factor; Yeast.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Forkhead Transcription Factors / genetics*
  • Forkhead Transcription Factors / metabolism
  • Gene Deletion
  • Gene Expression Regulation, Fungal
  • Gene Knockdown Techniques
  • Genes, Fungal
  • Metabolome
  • Minichromosome Maintenance 1 Protein / genetics*
  • Minichromosome Maintenance 1 Protein / metabolism
  • Saccharomyces cerevisiae / cytology*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins / genetics*
  • Saccharomyces cerevisiae Proteins / metabolism
  • Shelterin Complex
  • Telomere-Binding Proteins / genetics*
  • Telomere-Binding Proteins / metabolism
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism


  • DNA-Binding Proteins
  • FHL1 protein, S cerevisiae
  • Forkhead Transcription Factors
  • MCM1 protein, S cerevisiae
  • Minichromosome Maintenance 1 Protein
  • RAP1 protein, S cerevisiae
  • REB1 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Shelterin Complex
  • Telomere-Binding Proteins
  • Transcription Factors