RiboTALE: A modular, inducible system for accurate gene expression control

Navneet Rai; Aura Ferreiro; Alexander Neckelmann; Amy Soon; Andrew Yao; Justin Siegel; Marc T Facciotti; Ilias Tagkopoulos

doi:10.1038/srep10658

RiboTALE: A modular, inducible system for accurate gene expression control

Sci Rep. 2015 May 29:5:10658. doi: 10.1038/srep10658.

Authors

Navneet Rai¹, Aura Ferreiro², Alexander Neckelmann², Amy Soon², Andrew Yao³, Justin Siegel⁴, Marc T Facciotti³, Ilias Tagkopoulos⁵

Affiliations

¹ UC Davis Genome Center, University of California-Davis, Davis, CA, USA.
² UC Davis Undergraduate Program, University of California-Davis, Davis, CA, USA.
³ 1] UC Davis Genome Center, University of California-Davis, Davis, CA, USA [2] Department of Biomedical Engineering, University of California-Davis, Davis, CA, USA.
⁴ 1] UC Davis Genome Center, University of California-Davis, Davis, CA, USA [2] Department of Biochemistry &Molecular Medicine, University of California-Davis, Davis, CA, USA [3] Department of Chemistry, University of California-Davis, Davis, CA, USA.
⁵ 1] UC Davis Genome Center, University of California-Davis, Davis, CA, USA [2] Department of Computer Science, University of California-Davis, Davis, CA, USA.

Abstract

A limiting factor in synthetic gene circuit design is the number of independent control elements that can be combined together in a single system. Here, we present RiboTALEs, a new class of inducible repressors that combine the specificity of TALEs with the ability of riboswitches to recognize exogenous signals and differentially control protein abundance. We demonstrate the capacity of RiboTALEs, constructed through different combinations of TALE proteins and riboswitches, to rapidly and reproducibly control the expression of downstream targets with a dynamic range of 243.7 ± 17.6-fold, which is adequate for many biotechnological applications.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Biotechnology / instrumentation*
Biotechnology / methods
Gene Expression Regulation*
Gene Regulatory Networks*
Genes, Synthetic*