Structural elucidation of a polysaccharide from Chrysanthemum morifolium flowers with anti-angiogenic activity

Int J Biol Macromol. 2015 Aug;79:674-80. doi: 10.1016/j.ijbiomac.2015.04.026. Epub 2015 May 27.

Abstract

The flowers of Chrysanthemum morifolium were extracted with 5% sodium hydroxide, yielding a neutral polysaccharide (JHB0S2) with a molecular weight of 16 kDa by anion-exchange chromatography on a DEAE-cellulose column and gel permeation chromatography on a Sephacryl S-200 HR column. JHB0S2 was found to contain glucose, xylose, arabinose and galactose in molar ratio of 2.9:2.3:1.0:1.2. Through the linkage analysis, partial acid hydrolysis and NMR spectra and so forth, indicated that its backbone consisted of 1,4-linked β-Glcp, nearly 50% of which was substituted at O-6 by 1,2-linked α-Xylp terminated with Galp or Xylp. Furthermore, some of 1,2-linked α-Xylp was substituted at O-4 by α-Araf-(1→5)-α-Araf-(1→6)-β-Galp. The biological test showed that JHB0S2 could inhibit the tube formation of HMEC-1 cells.

Keywords: Anti-angiogenesis; Chrysanthemum morifolium; Polysaccharide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiogenesis Inhibitors / chemistry*
  • Angiogenesis Inhibitors / isolation & purification
  • Angiogenesis Inhibitors / pharmacology
  • Arabinose / analysis
  • Carbohydrate Sequence
  • Cell Line, Transformed
  • Cell Proliferation / drug effects
  • Chromatography, Gel
  • Chromatography, Ion Exchange
  • Chrysanthemum / chemistry*
  • Endothelial Cells / cytology
  • Endothelial Cells / drug effects
  • Flowers / chemistry*
  • Galactose / analysis
  • Glucose / analysis
  • Humans
  • Hydrolysis
  • Molecular Sequence Data
  • Molecular Weight
  • Neovascularization, Physiologic / drug effects*
  • Polysaccharides / chemistry*
  • Polysaccharides / isolation & purification
  • Polysaccharides / pharmacology
  • Sodium Hydroxide / chemistry
  • Xylose / analysis

Substances

  • Angiogenesis Inhibitors
  • Polysaccharides
  • Sodium Hydroxide
  • Xylose
  • Arabinose
  • Glucose
  • Galactose