Actinospica Durhamensis Sp. Nov., Isolated From a Spruce Forest Soil

Antonie Van Leeuwenhoek. 2015 Aug;108(2):435-42. doi: 10.1007/s10482-015-0496-1. Epub 2015 May 31.

Abstract

Seven acidophilic actinobacteria isolated from humus and mineral layers of a spruce forest soil were examined using a polyphasic approach. Chemotaxonomic properties of the isolates were found to be consistent with their classification in the genus Actinospica. The strains formed a distinct phyletic line in the Actinospica 16S rRNA gene tree being most closely related to Actinospica robiniae DSM 44927(T) (98.7-99.3 % similarity). DNA:DNA relatedness between isolate CSCA57(T) and the type strain of A. robiniae was found to be low at 40.8 (±6.6) %. The isolates were shown to have many phenotypic properties in common and were distinguished readily from the type strains of Actinospica acidiphila and A. robiniae using a range of phenotypic features. On the basis of these data the seven isolates were considered to represent a new species for which the name Actinospica durhamensis sp. nov. is proposed. The type strain of the species is CSCA 57(T) (=DSM 46820(T) = NCIMB 14953(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinobacteria / classification*
  • Actinobacteria / genetics
  • Actinobacteria / isolation & purification*
  • Actinobacteria / physiology
  • Bacterial Typing Techniques
  • Cluster Analysis
  • Culture Media / chemistry
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Forests
  • Hydrogen-Ion Concentration
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phylogeny
  • Picea
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Soil Microbiology*

Substances

  • Culture Media
  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/KJ445730
  • GENBANK/KJ445731
  • GENBANK/KJ445732
  • GENBANK/KJ445733
  • GENBANK/KJ445735
  • GENBANK/KJ445736
  • GENBANK/KJ445737