Functional differentiation of human pluripotent stem cells on a chip

Nat Methods. 2015 Jul;12(7):637-40. doi: 10.1038/nmeth.3411. Epub 2015 Jun 1.


Microengineering human "organs-on-chips" remains an open challenge. Here, we describe a robust microfluidics-based approach for the differentiation of human pluripotent stem cells directly on a chip. Extrinsic signal modulation, achieved through optimal frequency of medium delivery, can be used as a parameter for improved germ layer specification and cell differentiation. Human cardiomyocytes and hepatocytes derived on chips showed functional phenotypes and responses to temporally defined drug treatments.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Differentiation
  • Embryonic Stem Cells / cytology
  • Hepatocytes / cytology
  • Humans
  • Microfluidic Analytical Techniques / methods*
  • Myocytes, Cardiac / cytology
  • Pluripotent Stem Cells / cytology*