Chromosome Conformation Capture Carbon Copy (5C) in Budding Yeast

Cold Spring Harb Protoc. 2015 Jun 1;2015(6):593-8. doi: 10.1101/pdb.prot085191.

Abstract

Chromosome conformation capture carbon copy (5C) is a high-throughput method for detecting ligation products of interest in a chromosome conformation capture (3C) library. 5C uses ligation-mediated amplification (LMA) to generate carbon copies of 3C ligation product junctions using single-stranded oligonucleotide probes. This procedure produces a 5C library of short DNA molecules which represent the interactions between the corresponding restriction fragments. The 5C library can be amplified using universal primers containing the Illumina paired-end adaptor sequences for subsequent high-throughput sequencing.

MeSH terms

  • Carbon
  • Chromatin
  • Chromosome Mapping / methods
  • Chromosomes / metabolism*
  • DNA Primers / metabolism
  • Gene Library
  • Nucleic Acid Conformation*
  • Saccharomycetales / physiology*
  • Saccharomycetales / ultrastructure*

Substances

  • Chromatin
  • DNA Primers
  • Carbon