Opa1 overexpression ameliorates the phenotype of two mitochondrial disease mouse models

Cell Metab. 2015 Jun 2;21(6):845-54. doi: 10.1016/j.cmet.2015.04.016.


Increased levels of the mitochondria-shaping protein Opa1 improve respiratory chain efficiency and protect from tissue damage, suggesting that it could be an attractive target to counteract mitochondrial dysfunction. Here we show that Opa1 overexpression ameliorates two mouse models of defective mitochondrial bioenergetics. The offspring from crosses of a constitutive knockout for the structural complex I component Ndufs4 (Ndufs4(-/-)), and of a muscle-specific conditional knockout for the complex IV assembly factor Cox15 (Cox15(sm/sm)), with Opa1 transgenic (Opa1(tg)) mice showed improved motor skills and respiratory chain activities compared to the naive, non-Opa1-overexpressing, models. While the amelioration was modest in Ndufs4(-/-)::Opa1(tg) mice, correction of cristae ultrastructure and mitochondrial respiration, improvement of motor performance and prolongation of lifespan were remarkable in Cox15(sm/sm)::Opa1(tg) mice. Mechanistically, respiratory chain supercomplexes were increased in Cox15(sm/sm)::Opa1(tg) mice, and residual monomeric complex IV was stabilized. In conclusion, cristae shape amelioration by controlled Opa1 overexpression improves two mouse models of mitochondrial disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Electron Transport Complex I / genetics
  • Electron Transport Complex I / metabolism
  • Electron Transport Complex IV / genetics
  • Electron Transport Complex IV / metabolism
  • GTP Phosphohydrolases / biosynthesis*
  • GTP Phosphohydrolases / genetics
  • Gene Expression Regulation, Enzymologic*
  • Mice
  • Mice, Knockout
  • Mitochondria / enzymology*
  • Mitochondria / genetics
  • Mitochondria / pathology
  • Mitochondrial Diseases / enzymology*
  • Mitochondrial Diseases / genetics
  • Mitochondrial Diseases / pathology
  • Oxygen Consumption*


  • Ndufs4 protein, mouse
  • Electron Transport Complex IV
  • GTP Phosphohydrolases
  • Opa1 protein, mouse
  • Electron Transport Complex I