Belonidae is a good model for investigating speciation and biogeography. To obtain basic information on the phylogeny of Belonidae, we determined the complete mitogenome of Strongylura anastomella using next-generation sequencing. The complete mitogenome is 16,534 bp in length and consists of 2 rRNAs, 22 tRNAs, 13 protein-coding genes and a control region. The nucleotide composition is 31.1% A, 29.8% T, 14.5% G and 24.6% C, with an AT bias (60.9%). The gene direction and position were similar to those of other Beloniformes. Belonidae and Scomberesocidae were separated in the phylogenetic tree based on complete mitogenomes. Further study is required to clarify the phylogenetic relationships among Belonidae and families within Beloniformes.
Keywords: Belonidae; Beloniformes; Strongylura anastomella; mitochondrial genome.