Emerging fluorescent protein technologies

Curr Opin Chem Biol. 2015 Aug;27:10-7. doi: 10.1016/j.cbpa.2015.05.001. Epub 2015 May 29.

Abstract

Fluorescent proteins (FPs), such as the Aequorea jellyfish green FP (GFP), are firmly established as fundamental tools that enable a wide variety of biological studies. Specifically, FPs can serve as versatile genetically encoded markers for tracking proteins, organelles, or whole cells, and as the basis for construction of biosensors that can be used to visualize a growing array of biochemical events in cells and tissues. In this review we will focus on emerging applications of FPs that represent unprecedented new directions for the field. These emerging applications include new strategies for using FPs in biosensing applications, and innovative ways of using FPs to manipulate protein function or gene expression.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Biosensing Techniques / methods*
  • Fluorescent Dyes / chemistry*
  • Green Fluorescent Proteins / chemistry*
  • Green Fluorescent Proteins / genetics
  • Molecular Imaging / methods*
  • Optogenetics / methods*
  • Protein Binding

Substances

  • Fluorescent Dyes
  • green fluorescent protein, Aequorea victoria
  • Green Fluorescent Proteins