Enzymatic Decoration of Prebiotic Galacto-oligosaccharides (Vivinal GOS) with Sialic Acid Using Trypanosoma cruzi trans-Sialidase and Two Bovine Sialoglycoconjugates as Donor Substrates

J Agric Food Chem. 2015 Jul 1;63(25):5976-84. doi: 10.1021/acs.jafc.5b01505. Epub 2015 Jun 22.


Decoration of prebiotic galacto-oligosaccharides (GOS) with sialic acid yields mixtures of GOS and sialylated GOS (Sia-GOS), novel products that are expected to have both prebiotic and antiadhesive functionalities. The recombinantly produced trans-sialidase enzyme from Trypanosoma cruzi (TcTS), an enzyme with the ability to transfer (α2-3)-linked sialic acid from sialogalactoglycans to asialogalactoglycans, was employed to catalyze this sialylation. As sialic acid acceptor substrates, Vivinal GOS and derived fractions of specific degree of polymerization were taken. As sialic acid donor substrates, bovine κ-casein-derived glycomacropeptide [>99% N-acetylneuraminic acid (Neu5Ac); <1% N-glycolylneuraminic acid (Neu5Gc)] and bovine blood plasma glycoprotein mixture (45% Neu5Ac; 55% Neu5Gc) were selected, yielding potential food and feed products, respectively. High-pH anion-exchange chromatography, matrix-assisted laser-desorption ionization time-of-flight mass spectrometry, and nuclear magnetic resonance spectroscopy were used for product analysis.

Keywords: Trypanosoma cruzi; bovine blood plasma glycoprotein; bovine glycomacropeptide; galacto-oligosaccharides; sialic acid; trans-sialidase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caseins / chemistry*
  • Cattle
  • Color
  • Glycoconjugates / chemistry*
  • Glycopeptides / chemistry*
  • Glycoproteins / chemistry*
  • Glycoproteins / genetics
  • Glycoproteins / metabolism
  • Molecular Structure
  • N-Acetylneuraminic Acid / chemistry*
  • Neuraminidase / chemistry*
  • Neuraminidase / genetics
  • Neuraminidase / metabolism
  • Oligosaccharides / chemistry*
  • Prebiotics / analysis*
  • Protozoan Proteins / chemistry*
  • Protozoan Proteins / genetics
  • Protozoan Proteins / metabolism
  • Trypanosoma cruzi / enzymology


  • Caseins
  • Glycoconjugates
  • Glycopeptides
  • Glycoproteins
  • Oligosaccharides
  • Prebiotics
  • Protozoan Proteins
  • kappa-casein glycomacropeptide
  • trans-sialidase
  • Neuraminidase
  • N-Acetylneuraminic Acid