Optimization of the determination of tocopherols in Agaricus sp. edible mushrooms by a normal phase liquid chromatographic method

Lillian Barros; Daniela M Correia; Isabel C F R Ferreira; Paula Baptista; Celestino Santos-Buelga

doi:10.1016/j.foodchem.2008.03.016

Optimization of the determination of tocopherols in Agaricus sp. edible mushrooms by a normal phase liquid chromatographic method

Food Chem. 2008 Oct 15;110(4):1046-50. doi: 10.1016/j.foodchem.2008.03.016. Epub 2008 Mar 18.

Authors

Lillian Barros¹, Daniela M Correia², Isabel C F R Ferreira³, Paula Baptista², Celestino Santos-Buelga⁴

Affiliations

¹ CIMO-Escola Superior Agrária, Instituto Politécnico de Bragança, Campus de Sta. Apolónia, Apartado 1172, 5301-855 Bragança, Portugal; Unidad de Nutrición y Bromatología, Facultad de Farmacia, Universidad de Salamanca, Campus Miguel de Unamuno s/n, E-37007 Salamanca, Spain.
² CIMO-Escola Superior Agrária, Instituto Politécnico de Bragança, Campus de Sta. Apolónia, Apartado 1172, 5301-855 Bragança, Portugal.
³ CIMO-Escola Superior Agrária, Instituto Politécnico de Bragança, Campus de Sta. Apolónia, Apartado 1172, 5301-855 Bragança, Portugal. Electronic address: iferreira@ipb.pt.
⁴ Unidad de Nutrición y Bromatología, Facultad de Farmacia, Universidad de Salamanca, Campus Miguel de Unamuno s/n, E-37007 Salamanca, Spain.

PMID: 26047301
DOI: 10.1016/j.foodchem.2008.03.016

Abstract

Individual tocopherol profile of five Agaricus mushroom species, widely consumed in Portugal, was obtained by a normal-phase high-performance liquid chromatography (NP-HPLC). It was used a simple solid-liquid extraction procedure without saponification step and the chromatographic separation was achieved using a YMC-Pack Polyamine II column using an isocratic elution with hexane/ethyl acetate (70:30, v/v) at a flow rate of 1.0mL/min. The effluent was monitored by a fluorescence detector. All the compounds were separated in a period of time of 30min. The method proved to be sensitive, reproducible and accurate, allowing the determination of tocopherols.

Keywords: Analysis; HPLC; Mushrooms; Vitamin E.