A reliable phenotypic assay for detection of ESBLs and AmpCs in MBL-producing gram-negative bacteria with the use of aminophenylboronic acid, dipicolinic acid and cloxacillin

J Microbiol Methods. 2015 Aug:115:100-3. doi: 10.1016/j.mimet.2015.05.028. Epub 2015 Jun 3.

Abstract

ESBLs and AmpCs may escape detection when they coexist with metallo-β-lactamases such as New Delhi Metallo-β-lactamases-1. In this study a combination disk assay was established using cefotaxime, cefotaxime/clavulanic acid, cefotaxime/clavulanic acid/cloxacillin, cefoxitin and cefoxitin/phenylboronic acid/cloxacillin on Mueller Hinton agar supplemented with dipicolinic acid for determination of β-lactamases in the presence of NDM-1.

Keywords: Cloxacillin; Combination disk test; Dipicolinic acid; Gram-negative bacteria; NDM-1; Phenylboronic acid.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Bacterial Typing Techniques / methods*
  • Boronic Acids / pharmacology*
  • Cloxacillin / pharmacology*
  • Gram-Negative Bacteria / drug effects*
  • Gram-Negative Bacteria / enzymology
  • Gram-Negative Bacteria / genetics
  • Gram-Negative Bacteria / isolation & purification
  • Gram-Negative Bacterial Infections / microbiology
  • Humans
  • Phenotype
  • Picolinic Acids / pharmacology*
  • beta-Lactamases / genetics
  • beta-Lactamases / metabolism*

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Boronic Acids
  • Picolinic Acids
  • 3-aminobenzeneboronic acid
  • beta-Lactamases
  • Cloxacillin
  • dipicolinic acid