Analysis of ginsenosides in Panax ginseng in high pressure microwave-assisted extraction

Yutang Wang; Jingyan You; Yong Yu; Chenling Qu; Huarong Zhang; Lan Ding; Hanqi Zhang; Xuwen Li

doi:10.1016/j.foodchem.2008.01.028

Analysis of ginsenosides in Panax ginseng in high pressure microwave-assisted extraction

Food Chem. 2008 Sep 1;110(1):161-7. doi: 10.1016/j.foodchem.2008.01.028. Epub 2008 Jan 29.

Authors

Yutang Wang¹, Jingyan You¹, Yong Yu¹, Chenling Qu¹, Huarong Zhang¹, Lan Ding¹, Hanqi Zhang¹, Xuwen Li²

Affiliations

¹ College of Chemistry, Jilin University, Changchun 130012, PR China.
² College of Chemistry, Jilin University, Changchun 130012, PR China. Electronic address: analchem@jlu.edu.cn.

PMID: 26050179
DOI: 10.1016/j.foodchem.2008.01.028

Abstract

High pressure microwave assisted extraction (HPMAE) was applied to extract the ginsenosides from Panax ginseng root. The influences of extraction solvent, extraction pressure and extraction time were individually investigated. HPMAE has been compared with other extraction methods, including Soxhlet extraction, ultrasound-assisted extraction and heat reflux extraction. The determination of ginsenosides was performed by HPLC-ESI-MS. The results indicated that the HPMAE not only took a shorter time but also afforded higher extraction yields of ginsenosides, especially ginsenoside Rb1, Rc, Rb2 and Rd. Furthermore, the neutral ginsenosides and malonyl ginsenosides in Panax ginseng root extracts by HPMAE were investigated. The malonyl ginsenoside m-Rb1, m-Rc, m-Rb2 and m-Rd degraded in HPMAE at 400kPa (109-112°C) in 70%(v/v) ethanol-water and at 600kPa (112-115°C) in methanol, and transformed into corresponding neutral ginsenoside Rb1, Rc, Rb2 and Rd. Using water as extraction solution, the neutral ginsenosides degraded under HPMAE at 400kPa (135-140°C), and transformed into less polarity rare ginsenosides.

Keywords: Degradation; Ginsenoside; HPLC/MS; Microwave assisted extraction; Panax ginseng.