Glycosphingolipids are modulators of disease pathogenesis in amyotrophic lateral sclerosis

Proc Natl Acad Sci U S A. 2015 Jun 30;112(26):8100-5. doi: 10.1073/pnas.1508767112. Epub 2015 Jun 8.

Abstract

Recent genetic evidence suggests that aberrant glycosphingolipid metabolism plays an important role in several neuromuscular diseases including hereditary spastic paraplegia, hereditary sensory neuropathy type 1, and non-5q spinal muscular atrophy. Here, we investigated whether altered glycosphingolipid metabolism is a modulator of disease course in amyotrophic lateral sclerosis (ALS). Levels of ceramide, glucosylceramide, galactocerebroside, lactosylceramide, globotriaosylceramide, and the gangliosides GM3 and GM1 were significantly elevated in spinal cords of ALS patients. Moreover, enzyme activities (glucocerebrosidase-1, glucocerebrosidase-2, hexosaminidase, galactosylceramidase, α-galactosidase, and β-galactosidase) mediating glycosphingolipid hydrolysis were also elevated up to threefold. Increased ceramide, glucosylceramide, GM3, and hexosaminidase activity were also found in SOD1(G93A) mice, a familial model of ALS. Inhibition of glucosylceramide synthesis accelerated disease course in SOD1(G93A) mice, whereas infusion of exogenous GM3 significantly slowed the onset of paralysis and increased survival. Our results suggest that glycosphingolipids are likely important participants in pathogenesis of ALS and merit further analysis as potential drug targets.

Keywords: FALS; SALS; motor neuron disease; mouse models neurological disease.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amyotrophic Lateral Sclerosis / enzymology
  • Amyotrophic Lateral Sclerosis / physiopathology*
  • Animals
  • Disease Models, Animal
  • Disease Progression
  • G(M3) Ganglioside / administration & dosage
  • Glucosyltransferases / antagonists & inhibitors
  • Glycosphingolipids / physiology*
  • Humans
  • Injections, Intraventricular
  • Male
  • Mice
  • Mice, Transgenic
  • Spinal Cord / physiopathology
  • Superoxide Dismutase / genetics
  • Superoxide Dismutase / metabolism

Substances

  • G(M3) Ganglioside
  • Glycosphingolipids
  • Superoxide Dismutase
  • Glucosyltransferases
  • ceramide glucosyltransferase