Diacylglyceryltrimethylhomoserine content and gene expression changes triggered by phosphate deprivation in the mycelium of the basidiomycete Flammulina velutipes

Phytochemistry. 2015 Sep;117:34-42. doi: 10.1016/j.phytochem.2015.05.021. Epub 2015 Jun 5.

Abstract

Diacylglyceryltrimethylhomoserines (DGTS) are betaine-type lipids that are phosphate-free analogs of phosphatidylcholines (PC). DGTS are abundant in some bacteria, algae, primitive vascular plants and fungi. In this study, we report inorganic phosphate (Pi) deficiency-induced DGTS synthesis in the basidial fungus Flammulina velutipes (Curt.: Fr.) Sing. We present results of an expression analysis of the BTA1 gene that codes for betaine lipid synthase and two genes of PC biosynthesis (CHO2 and CPT1) during phosphate starvation of F. velutipes culture. We demonstrate that FvBTA1 gene has increased transcript abundance under phosphate starvation. Despite depletion in PC, both CHO2 and CPT1 were determined to have increased expression. We also describe the deduced amino acid sequence and genomic structure of the BTA1 gene in F. velutipes. Phylogenetic relationships between putative orthologs of BTA1 proteins of basidiomycete fungi are discussed.

Keywords: BTA1; Basidiomycetes; Betaine lipid; CHO2; CPT1; Flammulina velutipes; Gene expression; Glycerolipid metabolism; Phosphate starvation; Phylogenetic analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Basidiomycota / chemistry
  • Basidiomycota / genetics
  • Basidiomycota / metabolism*
  • Flammulina / metabolism*
  • Gene Expression
  • Glycolipids / analysis*
  • Glycolipids / metabolism
  • Molecular Sequence Data
  • Mycelium / drug effects*
  • Phosphates / analysis
  • Phosphates / metabolism
  • Phosphatidylcholines / analysis
  • Phylogeny
  • Triglycerides / analysis*
  • Triglycerides / genetics

Substances

  • 1(3),2-diacylglyceryl-3(1)-O-4'-(N,N,N-trimethyl)homoserine
  • Glycolipids
  • Phosphates
  • Phosphatidylcholines
  • Triglycerides