Abstract
Rv0880 from the pathogen Mycobacterium tuberculosis is classified as a MarR family protein in the Pfam database. It consists of 143 amino acids and has an isoelectric point of 10.9. Crystals of Rv0880 belonged to space group P1, with unit-cell parameters a = 54.97, b = 69.60, c = 70.32 Å, α = 103.71, β = 111.06, γ = 105.83°. The structure of the MarR family transcription regulator Rv0880 was solved at a resolution of 2.0 Å with an R(cryst) and R(free) of 21.2 and 24.9%, respectively. The dimeric structure resembles that of other MarR proteins, with each subunit comprising a winged helix-turn-helix domain connected to an α-helical dimerization domain.
Keywords:
MarR; MarR family protein; Mycobacterium tuberculosis; transcription factor.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Motifs
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Bacterial Proteins / chemistry*
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Bacterial Proteins / genetics
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Crystallization
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Crystallography, X-Ray
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DNA-Binding Proteins / chemistry*
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DNA-Binding Proteins / genetics
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Gene Expression
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Models, Molecular
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Molecular Sequence Data
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Mycobacterium tuberculosis / chemistry*
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Mycobacterium tuberculosis / metabolism
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Protein Multimerization
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Protein Structure, Secondary
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Protein Structure, Tertiary
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Structural Homology, Protein
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Transcription Factors / chemistry*
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Transcription Factors / genetics
Substances
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Bacterial Proteins
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DNA-Binding Proteins
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MarR-family transcriptional regulatory protein, Mycobacterium
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Recombinant Proteins
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Transcription Factors